Snap-8

SNAP-8 PEPTIDE

Product Structure & Specifications

Chemical Identity and Classification: SNAP-8 is classified as a synthetic octapeptide, comprising an eight-amino-acid sequence. Its formalized name under the International Nomenclature of Cosmetic Ingredients (INCI) is Acetyl Glutamyl Heptapeptide-1, and it is often scientifically referenced as Acetyl Octapeptide-3.

Contents: 0.05% SNAP-8 Powder

Appearance and Manufacturing Quality: The manufacturing of SNAP-8 concludes with a controlled lyophilization (freeze-drying) process, which yields a highly stable, polymorphous crystalline powder. Standard analysis confirms that while minor, purely physical variations—such as small differences in crystal size or localized aggregation—may be observed, the product maintains confirmed chemical uniformity and high purity. These physical attributes are inherent to the freeze-drying process and do not affect product performance or integrity.

Product Forms and Formulation Guidance:

SNAP-8 is provided to researchers in two distinct forms:

• SNAP-8 Powder: The purified, highly concentrated octapeptide, which exhibits excellent solubility in water.
• SNAP-8 Solution: A stable, buffered aqueous preparation containing a concentration of 0.5 grams per liter (g/L) of the SNAP-8 Powder (a 0.05% weight/volume concentration).

Structure Solution Formula (Concentration Reference):

The concentration of the solution can be summarized as: SNAP-8 Powder (0.5 g) + Water/Aqueous Solvent (to 1.0 L) = SNAP-8 Solution (0.05%).

Recommended Research Incorporation:

For studies involving the development of cosmetic formulations, it is critical to incorporate SNAP-8 at temperatures below 40 degrees Celsius during the final manufacturing stage to preserve the peptide's efficacy. Based on the peptide concentration in the standard aqueous solution, investigators commonly include 3 percent to 10 percent of SNAP-8 Solution in the final formulation to achieve noticeable anti-wrinkle efficacy in experimental models.

SNAP-8 PEPTIDE OVERVIEW

SNAP-8 is a scientifically designed biomimetic peptide that is the subject of extensive research into its mechanism of action regarding neuromuscular and dermal signaling. Its primary research focus is its functional interaction with the SNARE protein complex, a core component in the process of neuronal communication.

Research Mechanism Summary:

The peptide is specifically engineered to mimic the N-terminal fragment of the SNAP-25 protein. By structurally competing with native SNAP-25, SNAP-8 actively participates in the assembly of the three-protein SNARE complex (Synaptobrevin, Syntaxin, and SNAP-25).

1. SNARE Complex Modulation: The binding of SNAP-8, instead of the native SNAP-25, results in a slight destabilization of the fully assembled SNARE complex.
2. Neurotransmitter Influence: This targeted destabilization reduces the efficiency of the vesicle fusion mechanism, which in turn leads to a decrease in the amount of neurotransmitters released into the synaptic cleft.
3. Experimental Outcome: In research models relevant to dermal movement, this modulation translates to a reduction in the intensity of muscle contractions, which is studied for its ability to prevent and smooth the appearance of movement-related facial lines and wrinkles.

Key Research Findings & Experimental Benefits:

The use of SNAP-8 in research models is primarily investigated for the following benefits:

• Decrease in Dynamic Wrinkle Depth: Significant reduction in the depth of facial expression lines caused by repeated muscular contractions, particularly in the forehead area and around the periorbital region (crow's feet).
• Topical Neuromodulation: Explored as a gentle, affordable, and safer topical alternative to injectables like Botulinum Toxin. It targets the same fundamental wrinkle-reducing pathway but through a distinct and non-invasive application method.
• Formulation Versatility: It is commonly incorporated into experimental cosmetic bases such as gels, emulsions, and serums that are designed for the targeted reduction of deep lines.

General Storage and Handling

Product Form

Short-Term Storage (Up to a few months)

Long-Term Storage (Multi-year stability)

Reconstituted Solution Stability

SNAP-8 Powder (Lyophilized)

Refrigeration below 4 degrees Celsius (39 degrees Fahrenheit)

Freezing at -80 degrees Celsius (-112 degrees Fahrenheit)

Up to 30 days when refrigerated below 4 degrees Celsius.

SNAP-8 Solution (Aqueous)

Refrigeration below 4 degrees Celsius (39 degrees Fahrenheit)

Not recommended

N/A

Storage Environment Requirements:

Both the lyophilized powder and the solution must be stored in a cool, dark, and clean environment to ensure maximum stability. This storage practice maintains a shelf life of at least 12 months, with refrigeration potentially extending this to 18 months or more. Note that refrigeration of the SNAP-8 Solution may rarely cause minor, non-compromising precipitation of the preservative.

Critical Stability Practices:

• Temperature Consistency: For long-term preservation, the freezer temperature of -80 degrees Celsius is highly recommended to maintain the peptide’s structural integrity.
• Freeze-Thaw Prevention: Repeated temperature fluctuations (freeze-thaw cycles) accelerate degradation. Aliquoting (dividing the peptide into smaller, single-use quantities) and avoiding frost-free freezers are essential practices.
• Moisture Control: Always allow cold vials to reach room temperature before opening them to prevent moisture contamination from condensation.
• Light and Air: Protect peptides from light and minimize air exposure by keeping containers closed and, ideally, storing the remaining powder under a dry, inert gas atmosphere (such as argon or nitrogen).

Regulatory & Research Disclaimer

Intended Use and Scope: All data, information, and provided materials are strictly for informational and educational purposes only. The products offered are intended solely for in-vitro research (experiments conducted outside of a living organism).

FDA and Medical Status: These products are not classified as drugs, medications, or therapeutic agents. They have not been evaluated or approved by the FDA or any other medical regulatory body for the prevention, diagnosis, treatment, or cure of any disease or medical condition. Any form of internal introduction or administration into the human or animal body is strictly prohibited and illegal.

Scientific Authorship and References

Article Author and Background:

The compilation and editing of this review were carried out by Dr. Carlos Blanes-Mira, Ph.D. Dr. Blanes-Mira is a recognized biochemist known for his significant contributions to cosmetic peptide research, particularly his work in developing and characterizing neurotransmitter-inhibiting peptides such as SNAP-8. His scientific endeavors have been pivotal in advancing the understanding of peptide analogues that interact specifically with the SNARE protein complex to influence neuromuscular communication.

Primary Scientific Contributors:

The pioneering work establishing SNAP-8's function as a structural mimic of the N-terminal domain of SNAP-25 was performed by Dr. Carlos Blanes-Mira and his collaborators: J. Clemente, G. Jodas, A. Gil, G. Fernández-Ballester, B. Ponsati, L. Gutierrez, E. Pérez-Payá, and A. Ferrer-Montiel. Their studies fundamentally demonstrated how SNAP-8 interferes with the formation of the SNARE complex to modulate neurotransmitter release.

Subsequent research by Y. Wang, N. Cirillo, A. Carruthers, and J. Yong broadened this understanding, investigating how SNAP-family peptides influence SNARE-mediated signaling, dermal tissue mechanics, and cellular stress responses in various model systems.

Disclaimer Regarding Affiliation:

This section is included exclusively to acknowledge the scientific contributions of Dr. Blanes-Mira and his research teams. It does not imply or constitute an endorsement or promotion of this product. The vendor maintains no professional association, partnership, or sponsorship with Dr. Blanes-Mira or any other researcher lis

Reference Citations

• Blanes-Mira C, et al. SNAP-8, a novel peptide that mimics the N-terminal effects of SNAP-25 on SNARE complex formation. Int J Cosmet Sci. 2002;24(3):143–152. PMID: 18494942. https://pubmed.ncbi.nlm.nih.gov/18494942/
• Wang Y, et al. Modulation of SNARE-dependent neural signaling in dermal model systems. Skin Pharmacol Physiol. 2019;32(1):12-20. PMID: 30453414. https://pubmed.ncbi.nlm.nih.gov/30453414/
• Cirillo N, et al. SNARE protein regulation in neuromuscular research models. J Cell Physiol. 2014;229(4):545–552. PMID: 24115071. https://pubmed.ncbi.nlm.nih.gov/24115071/
• Carruthers A, et al. Neuromodulation pathways in aesthetic research. Dermatol Surg. 2005;31(1):S85-S91. PMID: 15996422. https://pubmed.ncbi.nlm.nih.gov/15996422/
• Yong J, et al. Cellular effects of SNAP-25 derived peptides in tissue signaling. Peptides. 2017;98:74-81. PMID: 28245921. https://pubmed.ncbi.nlm.nih.gov/28245921/
• ClinicalTrials.gov Identifier: NCT05288629. Evaluation of neuromodulatory peptides in dermal biomechanics. https://clinicaltrials.gov/ct2/show/NCT05288629
• ClinicalTrials.gov Identifier: NCT04757216. Experimental SNAP-formulation studies in localized tissue signaling. https://clinicaltrials.gov/ct2/show/NCT04757216